Chromatographic Tools in an mRNA process: From Analytics to Purification

Time: 10:00 am
day: Day One


  • Overcoming technical challenges at all stages: plasmid production and linearisation, in vitro transcription (IVT), purification of mRNA and production of lipid nanoparticles (LNP). In addition, improving process economics will further enable the use of mRNA therapeutics in low-income countries
  • Rapid, at-line analytical technologies can be used to monitor IVT reactions, and to optimise process parameters. Analytical HPLC with CIMac PrimaS™ can separate the constituents of the reaction, including the capping reagent, nucleotides, plasmid DNA, and mRNA within a 3-minute analysis time
  • Chromatography using multimodal ligands is well-established to capture non-polyadenylated RNA from IVT processes. CIM® PrimaS can separate RNA from contaminants in an IVT reaction. Multimodal chromatography is explored here as a capture method for mRNA purification